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An Integrated Microfluidic Chip for Synchronous Drug Loading, Separation and Detection of Plasma Exosomes

  • Date:2025-05-09
  • Hits:

  • Journal: 

    Lab on a Chip

  • Key Words: 

    Yu-Xin Zhang,‡ Ming Wang,‡ Li-Li Xu, Yi-Jing Chen, Shu-Ting Zhong, Ying Feng, d Hai-Bo Zhang,*Shi-Bo Cheng,* Min Xie* and Wei-Hua Huang， Accepted

  • Abstract: 

    Exosomes have gained increasing attention as robust, biocompatible carriers for targeted therapy. However, current
    techniques for exosome drug loading suffer from low drug loading efficiency, substantial exosome loss during repeated
    purification and quantification processes. Here, we present an integrated microfluidic chip (IMC) that streamlines drug
    loading, separation, and electrochemical detection of exosomes from plasma in a single device. In this design, the three
    dimensional (3D) macroporous scaffold and the magnetoresponsive electrode are successfully assembled into the modeling
    microchip, playing the functions of “3D chaotic flow mixer”, “magnetic separator” and “electrochemical detector”. When
    plasma, doxorubicin (DOX), boron clusters and immunomagnetic nanoprobes (IMP) are simultaneously injected into the
    IMC, the exosomes are loaded with DOX-boron cluster (EDB) complexes and synchronously recognized by IMP in the “3D
    chaotic flow mixer”. Our strategy exhibits high DOX loading efficiency owing to the superchaotropic effect of boron cluster
    and enhanced immunolabeling efficiency by the thorough mixing of 3D scaffold. Meanwhile, the novel magnetoresponsive
    electrode enables magnetic separation and real-time, enzyme-linked immunoelectrochemical quantification of exosomes,
    thereby simplifying the workflow from drug loading to quantification. The resulting EDB in combination with magnetic
    hyperthermia achieves up to 90% cell-killing efficiency against DOX-resistant breast cancer cells. Overall, our system could
    simultaneously realize the enhanced DOX loading into exosomes, efficient magnetic immunoseparation of exosomes, and
    sensitive electrochemical quantification of exosomes, offering a promising approach for autologous exosome-based drug
    delivery for cancer treatment.

  • Indexed by: 

    Journal paper

  • Discipline: 

    Natural Science

  • Document Type: 

    J

  • Translation or Not: 

    no

  • Date of Publication: 

    2025-05-09

  • Included Journals: 

    SCI
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