DOI码：10.1007/s10337-023-04235-6

发表刊物：Chromatographia

关键字：Coordination · Hydrophilic interaction liquid chromatography · Nickel oxide deposited silica · Stationary phase

摘要：Nickel oxide deposited silica stationary phase was prepared by liquid phase deposition and characterized. Its chromatographic
performance was evaluated using several compounds such as typical polar compounds and benzimidazoles as probes. The
effects of mobile phase variables such as acetonitrile content, salt concentration, and pH on the chromatographic behavior,
thermodynamics, and kinetics of these compounds were investigated to reveal the retention mechanism. The results showed that the prepared stationary phase exhibited a retention behavior of hydrophilic interaction liquid chromatography, and that
multiple retention mechanisms including partitioning, adsorption, ion-exchange, electrostatic attraction, and coordination
interactions contributed to solute retention. The coordination of nickel oxide electron-accepting sites and the electrondonating solutes resulted in the mixed-mode retention on stationary phase, which could be very useful for enhancing the chromatographic selectivity for the analytes. The batch-to-batch reproducibility was acceptable with the relative standard deviations of probe retention of less than 9.89%. The prepared nickel oxide deposited silica stationary phase was successfully employed for the separation of several compounds, and it showed better separation effect and different selectivity from silica column and commercial Zorbax NH2 column.

合写作者：Yingjie Guo, Xitian Peng*, Qiongwei Yu*, Yuqi Feng*

论文类型：期刊论文

学科门类：理学

文献类型：J

卷号：86

页面范围：125-134

是否译文：否

发表时间：2023-01-18

收录刊物：SCI

发布期刊链接：https://doi.org/10.1007/s10337-023-04235-6

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