Proteolytic regulation of a galectin-3/Lrp1 axis controls osteoclast-mediated bone resorption
发布时间：2024-10-26
点击次数：
- DOI码：
- 10.1083/jcb.202206121
- 发表刊物：
- J Cell Biol
- 摘要：
- Bone-resorbing osteoclasts mobilize proteolytic enzymes belonging to the matrix metalloproteinase (MMP) family to directly degrade type I collagen, the dominant extracellular matrix component of skeletal tissues. While searching for additional MMP substrates critical to bone resorption, Mmp9/Mmp14 double-knockout (DKO) osteoclasts-as well as MMP-inhibited human osteoclasts-unexpectedly display major changes in transcriptional programs in tandem with compromised RhoA activation, sealing zone formation and bone resorption. Further study revealed that osteoclast function is dependent on the ability of Mmp9 and Mmp14 to cooperatively proteolyze the beta-galactoside-binding lectin, galectin-3, on the cell surface. Mass spectrometry identified the galectin-3 receptor as low-density lipoprotein-related protein-1 (Lrp1), whose targeting in DKO osteoclasts fully rescues RhoA activation, sealing zone formation and bone resorption. Together, these findings identify a previously unrecognized galectin-3/Lrp1 axis whose proteolytic regulation controls both the transcriptional programs and the intracellular signaling cascades critical to mouse as well as human osteoclast function.
- 合写作者：
- Tang Y, Li XY, Kerk SA, Lyssiotis CA, Sun X, Wang Z, Cho JS, Ma J
- 第一作者：
- Zhu L
- 论文类型：
- 期刊论文
- 通讯作者：
- Weiss SJ
- 卷号：
- 222
- 期号：
- 4
- 页面范围：
- e202206121
- 是否译文：
- 否
- 发表时间：
- 2023-04-03
- 收录刊物：
- SCI