Feng Yong
+
Personal Information
- Supervisor of Master's Candidates
- Name (Pinyin):Feng Yong
- E-Mail:yongfeng@whu.edu.cn
- Education Level:研究生毕业
- Business Address:基础医学院1号楼
- Gender:Male
- Contact Information:18627962533； yongfeng@whu.edu.cn
- Professional Title:Associate professor
- Discipline:
Pathogenic Organisms;
Microbiology
Other Contact Information
- ZipCode:430071
- PostalAddress:武汉市武昌区东湖路115号，武汉大学基础医学院一号楼
- OfficePhone:027-68759997
- Telephone:18627962533
- email:yongfeng@whu.edu.cn
A comprehensive analysis of precursor microRNA cleavage by human Dicer
- Date of Publication:2018-10-14
- Hits:
Journal:
RNAKey Words:
Dicer, pre-miRNA, RNA binding, RNA processing, secondary structureAbstract:
Dicer cleaves double-stranded RNAs (dsRNAs) or precursor microRNAs (pre-miRNAs) to yield ∼22-nt RNA duplexes. The pre-miRNA structure requirement for human Dicer activity is incompletely understood. By large-scale in vitro dicing assays and mutagenesis studies, we showed that human Dicer cleaves most, although not all, of the 161 tested human pre-miRNAs efficiently. The stable association of RNAs with Dicer, as examined by gel shift assays, appears important but is not sufficient for cleavage. Human Dicer tolerates remarkable structural variation in its pre-miRNA substrates, although the dsRNAIndexed by:
Journal paperCorrespondence Author:
Zeng YanDiscipline:
Natural ScienceFirst-Level Discipline:
BiologyVolume:
18Issue:
11Page Number:
2083-2092ISSN No.:
1355-8382Translation or Not:
noDate of Publication:
2012-11-01Links to published journals:
https://rnajournal.cshlp.org/content/18/11/2083.long