A comprehensive analysis of precursor microRNA cleavage by human Dicer
发布时间：2018-10-14 点击次数：
发表刊物：RNA-A PUBLICATION OF THE RNA SOCIETY
关键字：Dicer, pre-miRNA, RNA binding, RNA processing, secondary structure
摘要：Dicer cleaves double-stranded RNAs (dsRNAs) or precursor microRNAs (pre-miRNAs) to yield ∼22-nt RNA duplexes. The pre-miRNA structure requirement for human Dicer activity is incompletely understood. By large-scale in vitro dicing assays and mutagenesis studies, we showed that human Dicer cleaves most, although not all, of the 161 tested human pre-miRNAs efficiently. The stable association of RNAs with Dicer, as examined by gel shift assays, appears important but is not sufficient for cleavage. Human Dicer tolerates remarkable structural variation in its pre-miRNA substrates, although the dsRNA
论文类型：期刊论文
通讯作者：曾严
学科门类：理学
一级学科：生物学
卷号：18
期号：11
页面范围：2083-2092
ISSN号：1355-8382
是否译文：否
发表时间：2012-11-01
